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Image Search Results
Journal: Cellular and Molecular Bioengineering
Article Title: An Easy-to-Fabricate Cell Stretcher Reveals Density-Dependent Mechanical Regulation of Collective Cell Movements in Epithelia
doi: 10.1007/s12195-021-00689-6
Figure Lengend Snippet: Schematic and computational analysis of a low-cost, easy-to-fabricate, pneumatically controlled uniaxial cell stretching device. (a) Schematic illustrations of the uniaxial stretch device in the cut-away side view before stretching (left) and after stretching (center) and the top view of the device (right). When vacuum pressure is applied to the two side vacuum chambers, the side chamber walls are deflected outward from the cell-culture chamber, resulting in the suspended silicone membrane being stretched. The stretching direction is perpendicular to the long axis of the cell-culture chamber. (b) Finite element analysis (FEA) example of the uniaxial stretch device before and after application of vacuum pressure to the side chambers. The color intensity indicates nodal strain calculated in the lateral stretch direction. Without applying vacuum pressure, no strain is applied to the membrane in the cell-culture chamber (top). Upon applying a vacuum pressure of 70 kPa, the cell-culture membrane is predicted to undergo 19% strain. (bottom). (c) FEA prediction of the strain profile of the cell-culture membrane corresponding to the pressure applied to the vacuum chamber. At 37 kPa, the membrane of the vacuum chamber makes contact with the top of the vacuum chamber, modeled as a contact event in the FEA model. (d) FEA prediction of the strain profile of the cell-culture membrane along the long axis of the device with applications of vacuum pressures from 5 to 70 kPa, demonstrating the homogeneity of strain. Every 5 kPa is depicted with a line that follows the strain (%) of the device along the long axis of the device.
Article Snippet: The
Techniques: Cell Culture, Membrane